Dinesh K. Singh
- PhD, Veterinary Pathology, Indian Veterinary Research Institute, Izzatnagar, India
- MVSc,Veterinary Pathology, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Nainital UP India
- BVSc and AH, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Nainital UP India
Research and Project Interests
- For more than 16 years, I have been fascinated by lentiviruses. I started working with Caprine Arthritis and Encephalitis virus and moved on to SIV, SHIV and HIV. My initial focus was to understand the role of primate lentiviral accessory genes on their pathogenicity using SHIV-macaque model. My experience was extended to research the development of a vaccine for HIV/AIDS. The current research focus in my lab is to test adjuvant activity of certain biomolecules to potentiate DNA vaccines for AIDS.
Minardi da Cruz JC, Singh DK, Lamara A, Chebloune Y. 2013. Small ruminant lentiviruses (SRLVs) break the species barrier to acquire new host range. Viruses. 2013 Jul 23;5(7):1867-84. doi: 10.3390/v5071867.
Lara HH, Ixtepan-Turrent L, Garza Treviño EN, Singh DK. 2011. Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins. J Nanobiotechnology. 2011 Sep 18;9:38. doi: 10.1186/1477-3155-9-38.
Mahapatro A, Singh DK. 2011. Biodegradable Nanoparticles are Excellent Vehicle for Site Directed in-vivo Delivery of Drugs and Vaccines. J Nanobiotechnology. 2011 Nov 28;9(1):55. PMID: 22123084
Lara HH, Ixtepan-Turrent L, Garza Treviño EN, Singh DK. 2011. Use of silver
nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins. J Nanobiotechnology. 2011 Sep 18;9:38. PMID: 21923937.
Lara HH, Garza-Treviño EN, Ixtepan-Turrent L, Singh DK. 2011. Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotechnology. 2011 Aug 3;9:30. PMID:21812950
Arrode G, Hegde R, Jin Y, Singh DK, Narayan O, Chebloune Y. Nef modulates the immunogenicity of Gag encoded in a non-infectious HIV DNA vaccine. Vaccine. 2008 Jul 23;26(31):3795-804.
Kumar A, Liu Z, Sheffer D, Smith M, Singh DK, Buch S, Narayan O. Protection of macaques against AIDS with a live attenuated SHIV vaccine is of finite duration. Virology. 2008 Feb 20;371(2):238-45.
Liu Z, Singh DK, Sheffer D, Smith MS, Dhillon S, Chebloune Y, Hegde R, Buch S, Narayan O. Immunoprophylaxis against AIDS in macaques with a lentiviral DNA vaccine. Virology. 2006 Apr 28.
Hegde R, Liu Z, Mackay G, Smith M, Chebloune Y, Narayan O, Singh DK. Antigen expression kinetics and immune responses of mice immunized with noninfectious simian-human immunodeficiency virus DNA. J Virol. 2005 Dec;79 (23):14688-97.
Singh DK, Liu Z, Sheffer D, Mackay GA, Smith M, Dhillon S, Hegde R, Jia F, Adany I, Narayan O. A noninfectious simian/human immunodeficiency virus DNA vaccine that protects macaques against AIDS. J Virol. 2005 Mar;79(6):3419-28.
Singh DK, Griffin DM, Pacyniak E, Jackson M, Werle MJ, Wisdom B, Sun F, Hout DR, Pinson DM, Gunderson RS, Powers MF, Wong SW, Stephens EB. The presence of the casein kinase II phosphorylation sites of Vpu enhances the CD4(+) T cell loss caused by the simian-human immunodeficiency virus SHIV(KU-lbMC33) in pig-tailed macaques. Virology. 2003 Sep1;313(2):435-51.
Mackay GA, Liu Z, Singh DK, Smith MS, Mukherjee S, Sheffer D, Jia F, Adany I, Sun KH, Dhillon S, Zhuge W, Narayan O. Protection against late-onset AIDS in macaques prophylactically immunized with a live simian HIV vaccine was dependent on persistence of the vaccine virus. J Immunol. 2004 Sep 15;173(6):4100-7.
Singh DK, Chouduri R, Pacyniak E, Berman NE, Stephens EB. Infection of human astrocytoma cells with simian-human immunodeficiency virus results in up-regulation of gene expression and altered growth properties. Neurosci Lett. 2003 Apr 17;340(3):201-4.
Potula R, Dhillion N, Sui Y, Zien CA, Funa K, Pinson D, Mayo MS, Singh DK, Narayan O, Buch S. Association of platelet-derived growth factor-B chain with simian human immunodeficiency virus encephalitis. Am J Pathol. 2004 Sep;165(3):815-24.
Stephens EB, Singh DK, Kohler ME, Jackson M, Pacyniak E, Berman NE. The primary phase of infection by pathogenic simian-human immunodeficiency virus results in disruption of the blood-brain barrier. AIDS Res Hum Retroviruses. 2003 Oct;19(10):837-46.
Stephens EB, McCormick C, Pacyniak E, Griffin D, Pinson DM, Sun F, Nothnick W, Wong SW, Gunderson R, Berman NE, Singh DK. Deletion of the vpu sequences prior to the env in a simian-human immunodeficiency virus results in enhanced Env precursor synthesis but is less pathogenic for pig-tailed macaques. Virology. 2002 Feb 15;293(2):252-61.
Singh DK, McCormick C, Pacyniak E, Griffin D, Pinson DM, Sun F, Berman NE, Stephens EB. Pathogenic and nef-interrupted simian-human immunodeficiency viruses traffic to the macaque CNS and cause astrocytosis early after inoculation. Virology. 2002 Apr 25;296(1):39-51.
Singh DK, McCormick C, Pacyniak E, Lawrence K, Dalton SB, Pinson DM, Sun F, Berman NE, Calvert M, Gunderson RS, Wong SW, Stephens EB. A simian human immunodeficiency virus with a nonfunctional Vpu (deltavpuSHIV(KU-1bMC33)) isolated from a macaque with neuroAIDS has selected for mutations in env and nef that contributed to its pathogenic phenotype. Virology. 2001 Mar 30;282(1):123-40.
Stephens EB, Singh DK, Pacyniak E, McCormick C. Comparison of Vif sequences from diverse geographical isolates of HIV type 1 and SIV(cpz) identifies substitutions common to subtype C isolates and extensive variation in a proposed nuclear transport inhibition signal. AIDS Res Hum Retroviruses. 2001 Jan 20;17(2):169-77
McCormick-Davis C, Dalton SB, Singh DK, Stephens EB. Comparison of Vpu sequences from diverse geographical isolates of HIV type 1 identifies the presence of highly variable domains, additional invariant amino acids, and a signature sequence motif common to subtype C isolates. AIDS Res Hum Retroviruses. 2000 Jul 20;16(11):1089-95.
McCormick-Davis C, Dalton SB, Hout DR, Singh DK, Berman NE, Yong C, Pinson DM, Foresman L, Stephens EB. A molecular clone of simian-human immunodeficiency virus (DeltavpuSHIV(KU-1bMC33)) with a truncated, non-membrane-bound vpu results in rapid CD4(+) T cell loss and neuro-AIDS in pig-tailed macaques. Virology. 2000 Jun 20;272(1):112-26.
Singh DK, Chebloune Y, Mselli-Lakhal L, Karr BM, Narayan O. Ovine lentivirus-infected macrophages mediate productive infection in cell types that are not susceptible to infection with cell-free virus. J Gen Virol. 1999 Jun;80 ( Pt 6):1437-44.
Chebloune Y, Karr BM, Raghavan R, Singh DK, Leung K, Sheffer D, Pinson D, Foresman L, Narayan O. Neuroinvasion by ovine lentivirus in infected sheep mediated by inflammatory cells associated with experimental allergic encephalomyelitis. J Neurovirol. 1998 Feb;4(1):38-48.
Stephens EB, Mukherjee S, Sahni M, Zhuge W, Raghavan R, Singh DK, Leung K, Atkinson B, Li Z, Joag SV, Liu ZQ, Narayan O. A cell-free stock of simian-human immunodeficiency virus that causes AIDS in pig-tailed macaques has a limited number of amino acid substitutions in both SIVmac and HIV-1 regions of the genome and has offered cytotropism. Virology. 1997 May 12;231(2):313-21.
Cedric S. Rodney Distinguished Service Award-2012
Wilveria Bass Atkinson Distinguished Research Award-2011
Bill Sheppard Master Teacher Award- 2011
Potentiating an HIV/DNA vaccine with Flagellin at Cervical Vaginal Mucosa- NIH/ NCMHD. Dinesh K. Singh (PI), Steven Mizel (Co-PI).
Nanoparticles and HIV. Dinesh K. Singh (PI).
Modulation of Dendritic Cells against HIV-1. Dinesh K. Singh (PI).
Team based teaching of advanced pathophysiology
Past teaching experiences (University of Kansas Medical
Virology (medical microbiology)
Diseases affecting central nervous system